منابع مشابه
Plasmid partition: sisters drifting apart.
Remarkably little is known about how bacterial chromosomes and their plasmids are partitioned to daughter cells prior to cell division, despite extensive experimentation and bioinformatic analysis. An in vitro study described in the current issue of The EMBO Journal recapitulates many features of plasmid partition in vivo and provides insight into how protein patterning on the nucleoid, determi...
متن کاملStructural biology of plasmid partition: uncovering the molecular mechanisms of DNA segregation.
DNA segregation or partition is an essential process that ensures stable genome transmission. In prokaryotes, partition is best understood for plasmids, which serve as tractable model systems to study the mechanistic underpinnings of DNA segregation at a detailed atomic level owing to their simplicity. Specifically, plasmid partition requires only three elements: a centromere-like DNA site and ...
متن کاملPlasmid segregation mechanisms.
Bacterial plasmids encode partitioning (par) loci that ensure ordered plasmid segregation prior to cell division. par loci come in two types: those that encode actin-like ATPases and those that encode deviant Walker-type ATPases. ParM, the actin-like ATPase of plasmid R1, forms dynamic filaments that segregate plasmids paired at mid-cell to daughter cells. Like microtubules, ParM filaments exhi...
متن کاملPlasmid partition system of the P1par family from the pWR100 virulence plasmid of Shigella flexneri.
P1par family members promote the active segregation of a variety of plasmids and plasmid prophages in gram-negative bacteria. Each has genes for ParA and ParB proteins, followed by a parS partition site. The large virulence plasmid pWR100 of Shigella flexneri contains a new P1par family member: pWR100par. Although typical parA and parB genes are present, the putative pWR100parS site is atypical...
متن کاملFine-structure analysis of the P1 plasmid partition site.
P1 plasmid partition requires two plasmid-encoded Par proteins and a cis-acting site. The site, parS, lies in a region consisting of a 13-bp palindrome and an adjacent AT-rich sequence. A series of point mutations were analyzed for their effects on partition site activity. The results indicated that only the left arm of the palindrome and some adjacent bases were needed. The limits of the funct...
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ژورنال
عنوان ژورنال: Microbiology Spectrum
سال: 2014
ISSN: 2165-0497
DOI: 10.1128/microbiolspec.plas-0023-2014